Stem Cell & iPSC Culture

Activin A (Act A) belongs to TGF- β Members of the family are dimeric proteins linked by disulfide bonds, originally extracted from the gonads and capable of stimulating the release of pituitary follicle stimulating hormone (FSH). Activin A has a wide range of biological activities and is involved in regulating many different functions, including hypothalamic and pituitary hormone secretion, gonadal hormone secretion, Germ cell development and maturation, red blood cell differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth. Recombinant Human Activin A is expressed by mammalian cells, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.

Epidermal Growth Factor (EGF) is capable of enhancing the proliferation and differentiation of mesenchymal cells and epithelial cells. In vitro, EGF plays a role of mitogen for fibroblasts, epithelial cells, and endothelial cells, facilitating colony formation in epithelial cell culture. In vivo, EGF promotes epithelial cell and blood vessel formation and inhibits gastric acid secretion. Recombinant Human EGF is produced by E.coli expression system, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.

Recombinant Human Fibroblast Growth Factor Basic (FGF basic or FGF2/bFGF) is a member of the FGF family. It is a heparin-binding growth factor secreted by endothelial cells, smooth muscle cells, and macrophages. It can promote the proliferation of a series of cells, including mesenchymal cells, neuroectoderm, vascular endothelial cells, and smooth muscle cells. FGF basic serves for angiogenesis regulation, wound healing and tissue repair, embryonic development and differentiation, neuronal protection, etc. Recombinant human FGF basic is expressed by E. coli , and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.

Fibroblast growth factor 8 (FGF-8) is a member of the fibroblast growth factor family. It has been found to be an essential growth factor for the androgen-dependent growth of mouse mammary cancer cells. Mouse FGF-8b has 100% amino acid identity with human FGF-8b. FGF-8 is widely expressed during embryonic development and mediates epithelial-mesenchymal transition. It plays a crucial role in regulating embryonic development, cell proliferation, cell differentiation, and cell migration. It is essential for the normal development of the brain, eyes, ears, limbs, and the normal development of the gonadotropin-releasing hormone (GnRH) neuronal system during embryogenesis. Recombinant Human FGF-8b is expressed by E.coli, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.

Fibroblast growth factor 9 (FGF-9) belongs to the fibroblast growth factor (FGF) family. Members of the FGF family have a broad range of mitogenic and cell survival activities and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF-9 plays a crucial role in regulating embryonic development, cell proliferation, cell differentiation, and cell migration. Additionally, FGF-9 may be involved in the growth and differentiation of glial cells during development, glial proliferation during brain tissue repair and regeneration after injury, differentiation and survival of neuronal cells, and stimulation of the growth of gliomas. Recombinant Human FGF-9 is expressed by E.coli, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.

Glial cell line-derived neurotrophic factor (GDNF) is a disulfide-linked homodimeric glycoprotein that belongs to the TGF-β superfamily. It has been shown to promote the survival of different subpopulations of neurons in both the central and peripheral nervous systems at various stages of development. Human GDNF cDNA encodes a 211 amino acid precursor, which is processed to produce the dimeric protein. Mature human GDNF is expected to contain two subunits, each with 134 amino acid residues. Cells known to express GDNF include supporting cells, type I astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. Mutations in this gene may be associated with congenital megacolon. Recombinant Human GDNF is expressed by E.coli, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.

Laminins are large molecular weight glycoproteins constituted by the assembly of three disulfide-linked polypeptides, the α, β and γ chains. Laminin regulates various cellular signaling pathways by binding to cell surface receptors, resulting in the production of more functional cells. Laminin can bind to integrins, polysaccharides, and other substances on the surface of cells. Among them, integrin α6β1 is the main cell adhesion receptor of Laminin and is highly expressed in embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Therefore, Laminin, as the main component of the basement membrane, can be used as a clear substrate for culturing ESCs and iPSCs without feeder layers. Recombinant Human Laminin521 E8 is expressed by mammalian cells, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.

Insulin-like growth factor 1 (IGF-1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contains the N- and C-terminal propeptide regions. Mature human IGF-1 consisting of 70 amino acids has 94% identity with mouse IGF-1 and exhibits cross-species activity. IGF-1 binds IGF-IR, IGF-IIR, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression. IGF-1 expression is regulated by growth hormone. R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg (R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. R3 IGF-1 has been produced with the purpose of increasing biological activity. R3 IGF-1 is significantly more potent than human IGF-1 in vitro. Recombinant Human LR3 Insulin-Like Growth Factor-1 is produced by E.coli expression system, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs), Noggin antagonizes the biological activity of BMP by blocking the binding of BMP to type I and II receptors. Noggin shows a high affinity to BMP4 but a low affinity to BMP7. During embryogenesis, Noggin antagonizes specific BMPs at defined times, for example, during neural tube, somite and cardiomyocyte growth and patterning. During bone development, Noggin prevents chondrocytes from proliferating so as to allow normal joint formation. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. Recombinant Human Noggin is expressed by mammalian cells, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.

R-Spondin 1 (RSPO1) is a secreted protein that contains two FU (furin like) repeat sequences and one TSP type I domain, belonging to the R-spondin family. Regardless of gender, RSPO1 is required for early development of Gonad. This phenomenon was discovered only 11 days after fertilization in mice. In order to induce cell proliferation, it collaborates with WNT4. They help stabilize β Chain proteins, thereby activating downstream targets. RSPO1 is necessary in female sexual development. It enhances WNT/ β The catenin pathway is used to combat male sexual development. During the critical gonadal stage, 6 to 9 weeks after fertilization, the ovaries are upregulated while the testes are downregulated. RSPO1 may help to treat mucositis characterized by oral inflammation. For cancer patients with head and neck tumors, they often suffer from this inflammatory pain with chemotherapy and radiation therapy. Recombinant Human RSPO1 is expressed by mammalian cells, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.

Transforming growth factor (TGF-beta 1 or TGF-β) is a multifunctional cytokine with an important regulatory effect on cell growth, differentiation, and immune function. It can both activate or inhibit the immune system. The tumor inhibitory effect of TGF-beta signaling derives from its ability to induce the expression of multiple genes. These genes are involved in inhibiting cell proliferation, inducing apoptosis, activating autophagy, inhibiting growth factor signaling through stromal fibroblasts, inhibiting inflammation, and inhibiting angiogenesis. Recombinant Human TGF-beta 1 is expressed by mammalian cells, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.

Vitronectin is a large glycoprotein found in blood and the extracellular matrix (ECM). The gene for Vitronectin encodes a 19 amino acid (aa) signal peptide and a 459 aa protein. The apparent molecular weight of human Vitronectin is 75 kDa, with ~30% of its molecular mass being attributed to glycosylation at 3 different sites. Vitronectin is involved in a number of biological activities including cell adhesion, cell spreading and migration, cell proliferation, extracellular anchoring, fibrinolysis, hemostasis, and complement mediated immune defense. It is worth noting that in the culture of pluripotent stem cells, due to the clear chemical composition of the protein, Recombinant Human Vitronectin helps to reduce the instability, compared with human plasma-derived Vitronectin and basement membrane extracts (BMEs). Recombinant Human Vitronectin is expressed by mammalian cells, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophic factor family. BDNF binds with high affinity to the TrkB kinase receptor, alongside other neurotrophic factors such as NGF, NT-3, and NT-4. It also binds to LNGFR (low-affinity nerve growth factor receptor, also known as p75). BDNF promotes the survival, growth, and differentiation of neurons. It is a major regulatory factor for synaptic transmission and plasticity in the adult central nervous system. The expression levels of BDNF are altered in neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Recombinant Human/Murine/Rat BDNF is expressed by E.coli, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.