mRNA Raw Materials & Reagents

By using T7 RNA polymerase for In Vitro Transcription (IVT), a large amount of messenger RNA (mRNA) can be prepared for mRNA vaccine drug development and other related studies. During this reaction, double-stranded RNA (dsRNA) were generated due to different reasons such as sequence structure, T7 RNA polymerase and preparation of reaction system. dsRNA is a pathogen-associated molecular pattern (pathogen-associated Molecular pattern) that can be recognized by multiple pattern receptors of cytoplasmic species, triggering activation of immune pathways, leading to translational inhibition and degradation of mRNA vaccines which affects the ultimate safety and effectiveness of the vaccine. Therefore, it is important to identify, quantify and control dsRNA in the process of mRNA vaccine development. This kit uses double antibody sandwich enzyme-linked immunoassay (ELISA) to detect dsRNA, which can detect and quantify dsRNA residue in mRNA with high sensitivity and specificity.

DNase I (Deoxyribonuclease I) was initially isolated from bovine pancreas. It can randomly degrade single stranded or double stranded DNA with equal efficiency, generating oligonucleotides with 5’-P. The bioactivity of DNase I is highly dependent on Ca²⁺, Mg²⁺ or Mn²⁺. In the presence of Mg²⁺, DNase randomly cuts dsDNA; in the presence of Mg²⁺, DNase cleaves both strands of dsDNA at approximately the same site, resulting in a blunt end or a sticky end with 1 or 2 protruding nucleotides.  DNase I is GMP Grade produced in Pichia pastoris. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has completed the DMF record of FDA and passed the HALAL certification.

Different grades and packaging are available.

Different grades and packaging are available.

Different grades and packaging are available.

With S-adenosylmethionine (SAM) as the methyl group base donor, mRNA Cap 2´-O-Methyltransferase can add a methyl group to the 2´-O of the first nucleotide of the Cap0 structure, [m7GpppN1(pN)x-OH(3')], next to the RNA 5´ end. Methylation of capped mRNA from Cap 0 to Cap1 naturally exists in eukaryocyte, where the Cap1 structure shows an important effect for translation, thus for expression as well. This product doesn’t show a reactivity to RNA substrate other than RNA with Cap0 structure. This product is from a large scale GMP leveled recombinant Cap 2’-O-Methyltransferase production via E. coli expression. Applying pharmaceutical leveled adjuvant and material for production, strictly controlling host protein residues, nucleic acid residues and other impurities, we guarantee manufacture and quality control practice complying to GMP regulation, as well as all the materials traceable. This product has completed the DMF record of FDA and passed the HALAL certification.

This product is used for pre-treatment of mRNA capping rate detection samples. Combined with special cleavage probe, 15-30nt samples with 5 'end cap structure can be obtained. This kit uses RNase H and cleavage probe to specifically cut the sample, complete the probe binding and enzyme digestion in one step, and effectively recover the 5 'end enzyme digestion fragment by using the specially optimized precipitation solution. The process is simple and rapid, and the treated sample can be directly used for capping rate detection with LC-MS system. The specially designed cleavage probe can effectively reduce the formation of secondary cleavage products and contribute to the analysis of subsequent capping rate detection results.

This product can be used for pretreatment of mRNA capping rate detection samples. Combined with special cleavage probe, 15-30nt sample restriction fragment with cap structure at 5 'end can be obtained. This kit uses RNase H and cleavage probe to specifically cleave samples, and the probe binding and digestion are completed in one step which is simple and rapid. The biotin-labeled cleavage probe can be adsorbed by streptavidin magnetic beads, and a single enzyme digestion product can be separated after purification. The specially designed cleavage probe can effectively reduce the formation of secondary cleavage products which is convenient for the subsequent analysis of the capping rate detection.

As the effective component in vaccines or drugs for injection into human, application of the raw materials for producing mRNA should comply with relevant terms of the current edition of " the Pharmacopoeia of the People’s Republic of China " and/or conform to general international common criteria. The identification test of the raw material is the confirmation test of the known raw material to confirm whether the raw material stored in the labelled container is the raw material as indicated. Various enzymes are used in the process of in vitro transcription and modification of mRNA. As a protein substance, enzymes can be identified by Dot Immunobinding Assay (DIBA, 2020 edition, Part IV, General Principles 3402). This kit uses polyvinylidene fluoride (PVDF) as the solid phase, carries out antigen-antibody reaction by DIBA, and carries out the identification test of various raw materials. The kit contains six antibodies, anti-T7 RNA Polymerase antibody, anti-Pyrophosphatase Inorganic (Yeast) antibody, anti-RNase Inhibitor antibody, anti-DNase I antibody, anti-Vaccinia Capping Enzyme antibody and anti-mRNA Cap 2´ -O-Methyltransferase antibody. Enzymes for in vitro transcription and modification of mRNA can be identified by specific qualitative analysis. The kit is easy to operate, clear in results, and has the characteristics of high specificity and high durability.

Inorganic Pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates: Applied in nucleic acid amplification experiments, PPase can hydrolyze the inorganic pyrophosphate generated by the polymerization to avoid its inhibition effect to synthesis of RNA strand. Pyrophosphatase, Inorganic (yeast) is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has completed the DMF record of FDA and passed the HALAL certification.

Inorganic Pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates: Applied in nucleic acid amplification experiments, PPase can hydrolyze the inorganic pyrophosphate generated by the polymerization to avoid its inhibition effect to synthesis of RNA strand. In the production process of mRNA vaccine drugs, PPase can increase the production amount of mRNA. According to regulations, various residues should be detected for mRNA vaccine solution, among which, PPase, as a protein product, belongs to the category of protein residue detection item, so quantitative detection of its residues should be carried out. This kit can detect and quantitatively analyze the PPase residues in Mrna solution with high sensitivity and specificity.

10×BsaI Reaction Buffer, GMP Grade is the companion reaction buffer for the restriction endonuclease BsaI.

10×BspQI Reaction Buffer, GMP Grade is the companion reaction buffer for the restriction endonuclease BspQI.

10×Capping Reaction Buffer, GMP Grade is the companion reaction buffer for the Vaccinia Capping Enzyme, GMP Grade and mRNA Cap 2´-O-Methyltransferase, GMP Grade

10×Poly(A) Polymerase Buffer, GMP Grade is the companion reaction buffer for the E. coli Poly(A) Polymerase, GMP Grade and ATP, GMP Grade

10×RNase R Buffer, GMP Grade is a matching reaction buffer for RNase R This product manufacturing processes are following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

10×Transcription Buffer, GMP Grade is the reaction buffer for T7 RNA Polymerase 2.0. This buffer has been optimized to produce 150-250μg of RNA in one reaction. It is also suitable for co-transcription systems, and the co-transcription capping effciency is greater than 95%. The synthesized RNA can be used for downstream applications such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation. 10×Transcription Buffer processes are strictly controlled to ensure the end products free from impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

10×XbaI Reaction Buffer, GMP Grade is a reaction buffer for restriction endonuclease XbaI This product is produced with pharmaceutical specifications of raw materials, and strictly control all kinds of pollution in the production process. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

ATP, short for Adenosine-5'-triphosphate, is an essential component of energy conversion in biological systems and can be used in a variety of related applications in molecular biology, such as kinase reactions, in vitro transcription, linking and phosphorylation. This product is an ATP sodium saline solution with purity ≥96% (HPLC) with a concentration of 10mM±2mM and is free from endonuclease, exonuclease and ribonuclease contamination. ATP processes are strictly controlled to ensure the end products free from impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has passed the HALAL certification.

Restriction endonucleases are a class of endonuclease that recognize specific deoxynucleotide sequences and cleave the phosphodiester bond between two deoxyribonucleotides at specific sites in each chain. BsaI derived from Bacillus Thermophilus, is a commonly used restriction endonuclease. This product is expressed from E. coli using gene recombination technology, our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

Restriction endonucleases are a class of endonuclease that recognize specific deoxynucleotide sequences and cleave the phosphodiester bond between two deoxyribonucleotides at specific sites in each chain. BsaI derived from Bacillus Thermophilus, is a commonly used restriction endonuclease. BsaI recognizes the following sequence: 5’…GGTCTC(N)1↓…3’; 3’…CCAGAG(N)5↑…5’. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has completed the DMF record of FDA.

Restriction endonucleases are a class of endonuclease that recognize specific deoxynucleotide sequences and cleave the phosphodiester bond between two deoxyribonucleotides at specific sites in each chain. BspQI derived from Bacillussphaericus, is a commonly used restriction endonuclease. BspQI recognizes the following sequence: 5’… GCTCTTC (N)1↓…3’ 3’… CGAGAAG (N)4↑…5’ Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has completed the DMF record of FDA.

Different grades and packaging are available.

Different grades and packaging are available.

E. coli Poly (A) Polymerase does not depend on the presence of template, and can catalyze the sequential incorporation of ATP in the form of AMP into the 3´ terminal of RNA, i.e., the addition of polyA tail to the 3´ terminal of RNA. Poly (A) polymerase has A high tail adding efficiency and can add 20 to 200 A bases to the 3´ terminal of RNA. Polyadenylation improves the stability of RNA in cells and enhances the expression efficiency of RNA after transfection or microinjection. Poly(A) tails can be used as primer binding sites for first-strand cDNA synthesis in some applications and can be used for end-labeling or quantification of miRNA. The original enzymes of T7 RNA Transcription Enzyme Mix produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

GTP, short for Guanosine-5'-triphosphate, can be used for a variety of related applications in molecular biology, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. In addition, GTP plays an important role in signal transduction as a phosphoric acid or pyrophosphoric acid messenger: GTP activates G protein, which induces various protein kinase-mediated cascades, leading to various cytological behaviors, such as cell proliferation and differentiation. GTP can also be used as a high energy precursor of single nucleotides and participate in the enzyme synthesis of DNA and RNA. This product is GTP sodium saline solution with purity ≥96% (HPLC), concentration is 10mM±2mM, and is free from endonuclease, exonuclease and ribonuclease contamination. GTP processes are strictly controlled to ensure the end products free from impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has passed the HALAL certification.

Different grades and packaging are available.

Different grades and packaging are available.

Different grades and packaging are available.

RNase Inhibitor can specifically inhibit the activities of RNase A, B, and C by forming a 1:1 complex with RNase, thereby suppressing its activity. During the production of mRNA vaccines, RNase Inhibitor effectively inhibits the potential RNase activity in the reaction system, protecting the mRNA from degradation. According to regulations, it is necessary to conduct various residue detections on the mRNA vaccine substances, among which RNase Inhibitor, as a protein product, falls into the category of protein residue detection items, so its residue amount needs to be quantitatively detected. This kit uses double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect RNase Inhibitor, which can detect and quantitatively analyze the content of RNase Inhibitor with high sensitivity and specificity.

RNase Inhibitor inhibits RNase A, B and C with high efficiency by forming a 1:1 complex with them. The characteristics of RNase Inhibitor are similar to those derived from pig liver and human placenta. The inhibition is reversible, as the complex can be dissociated by urea and sulfhydryl reagents in which RNase refolds and the inhibitor is inactivated. It can be directly added to the reaction containing RNA. Different from other competitive inhibitors (e.g., nucleic acids, inorganic phosphoric acids), this product is a protein, which can be easily removed from the reaction by phenol extraction. However, the reverse transcriptase RNase H could not be inhibited. Recombinant RNase Inhibitor is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has completed the DMF record of FDA and passed the HALAL certification.

Ribonuclease R (RNase R), from the RNR superfamily of E.coli, is a Mg²⁺-dependent 3'-5' exonuclease. RNA can be cleaved into dinucleotide and trinucleotide gradually from the 3'-5' direction by RNase R. RNase R can digest most of the linear RNA. However, it is difficult to digest circular RNA, lariat RNA and short double-stranded RNA molecules with less than 7 nucleotides of 3' end protrusion. RNase R is commonly used in gene expression and alternative splicing studies and digests linear RNA to enrich circular RNA or lariat RNA. This product is obtained using recombinant protein technology. Our manufacturing processes are following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

Ribonuclease T1 (RNase T1) is a recombinant protein obtained through a series of purification steps and is an endoribonuclease. This enzyme specifically cleaves single-stranded RNA at guanosine residues, producing 3' phosphate termini. The enzyme cleaves the phosphodiester bond between the 3'-guanosine residue and the adjacent nucleotide's 5'-OH group by forming a nucleoside 2',3'-cyclic phosphate intermediate. The reaction products are oligonucleotides with 3'-GMP termini. RNase T1 does not require metal ions for its activity and can be used for rapid analysis of the physical structure of target RNA. This product is strictly controlled for host nuclease residues, with high enzyme activity and strong specificity.

S-(5’-Adenosyl)-L-methionine (SAM, AdoMet) with an activating methyl group is the methyl donor for vaccinia capping enzyme and mRNA Cap 2’-O-Methyltransferase catalyzed methylation, the storage buffer formula is: 0.005M Sulfuric Acid, 10% ETOH. SAM processes are strictly controlled to ensure the end products free from impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has passed the HALAL certification.

Different grades and packaging are available.

Different grades and packaging are available.

As a biological macromolecule, mRNA can be synthesized on a large scale by in vitro transcription (IVT). T7 promoter is one of the most efficient promoters at present. Therefore, T7 RNA polymerase can be used for in vitro transcription to obtain more synthetic products. As one of the by-products of in vitro transcription, dsRNA can be recognized by the corresponding nucleic acid receptors, triggering a natural immune inflammatory response, which seriously affects the effectiveness of mRNA vaccines and needs to be strictly controlled during the production process. T7 RNA polymerase obtained through molecular evolution. It can significantly reduce the dsRNA impurities, thereby reducing the autoimmunogenicity of synthetic mRNA. The polymerase is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

T7 RNA Polymerase is a DNA-dependent 5'→3' RNA polymerase that highly specifically recognizes the T7 promoter sequence. It uses double-stranded DNA containing the T7 promoter sequence as a template and NTP as a substrate to synthesize RNA complementary to one strand of DNA. The transcribed RNA can be used for downstream applications such as RNA structure and function research, RNA enzyme protection, probe hybridization, RNAi, microinjection and in vitro translation. In the production process of mRNA drugs, according to regulations, it is necessary to conduct various residue detections on the mRNA vaccine substances, among which T7 RNA polymerase, as a protein product, falls into the category of protein residue detection items, so its residue amount needs to be quantitatively detected.

As a biological macromolecule, mRNA can be synthesized on a large scale by in vitro transcription (IVT). T7 promoter is one of the most efficient promoters at present. Therefore, T7 RNA polymerase can be used for in vitro transcription to obtain more synthetic products. T7 RNA polymerase is a T7 promoter-specific, DNA-dependent, 5'→3' RNA polymerase from T7 bacteriophage. Using double stranded DNA as the template, it transcribes RNA complementary to the single stranded DNA located at the downstream of T7 promoter. T7 RNA polymerase has been commonly used for in vitro mRNA synthesis. The polymerase is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has completed the DMF record of FDA and passed the HALAL certification.

As a biological macromolecule, mRNA can be synthesized on a large scale by in vitro transcription (IVT). T7 promoter is one of the promoters with the highest transcription efficiency. Therefore, T7 RNA Polymerase can be used for in vitro transcription to obtain more synthetic products. T7 RNA Transcription Enzyme Mix has been optimized by a series of transcription systems. One reaction can transcribe up to 150-200μg of RNA, and the synthesized RNA can be used downstream in many aspects such as mRNA vaccine preparation, RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation application. The original enzymes of T7 RNA Transcription Enzyme Mix produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has passed the HALAL certification.

Different grades and packaging are available.

Different grades and packaging are available.

The vaccinia capping enzyme together with substrates, is capable to add the 7-methylguanosine capping structure (m7Gppp, Cap0) to the 5´ end of the RNA. In eukaryotes, this structure is closely related to the stability, transport, and translation of mRNA. Capping RNA with an enzymatic reaction is a simple and effective method that can significantly improve the stability and translation capabilities of RNA used for in vitro transcription, transfection, and micro-injection. The vaccinia virus capping enzyme consists of two subunits (D1 and D12) (Figure 1a), and possesses RNA triphosphatase, guanosine transferase, and guanine methyltransferase activities, and they are all necessary for adding a complete Cap0 structure m7Gppp5´N to mRNA. This product can be applied to capping reaction of RNA generated by the T7 RNA Polymerase. The capping reaction is completed within an hour and the efficiency is close to 100% with correct direction. Vaccinia Capping Enzyme is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has completed the DMF record of FDA and passed the HALAL certification.

Restriction enzymes are a class of endonucleases that recognize specific deoxynucleotide sequences and cleave phosphodiester bonds between two deoxyribonucleotides at specific sites in each strand. Restriction enzymes are important components of the "restriction-modification system". The biological role is to protect the host from foreign DNA infection. XbaI, derived from Xanthomonas campestris, is a commonly used type II restriction enzyme. This product is obtained by recombinant protein production technology, using pharmaceutical specifications of raw materials production, and strict control of bacterial endotoxin, nucleic acid residues. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.