Biofarmaceutica

HEPES FREE ACID, GMP, EXCIPIENT GRADE is a zwitterionic buffer used to maintain pH of media used in cell cultures. It is one of Good’s twelve buffers that has a pKa value similar to its pH value making it an ideal buffer for pH maintenance. It has only one restriction, it interferes with the Folin protein assay. This buffer can form radicals so it is not suitable for redox studies. HEPES is used as a Good’s buffer because it has low UV absorptivity, minimal reactivity,stable pH and is soluble in water.

The core role of Ferric Ammonium Citrate in cell culture media is as an efficient and bioavailable iron ion supplement (iron source), while also providing a small amount of nitrogen source and participating in the regulation of cell metabolism and protein expression

L-CYSTINE DISODIUM SALT MONOHYDRATE are Mammalian cells are unable to synthesize sufficient amounts of cysteine/cysteine and require exogenous supplementation. It is reduced to L-cysteine inside the cell and directly participates in protein synthesis. It is an essential amino acid for cell growth and proliferation.

L-(-)-FUCOSE, LOW ENDOTOXIN the core role of fucose (mainly L-fucose) in cell culture is to regulate the fucosylation modification of glycoproteins, thereby affecting the function, cell signaling, adhesion, and differentiation of recombinant proteins (especially antibodies).