Restriction endonucleases are a class of endonuclease that recognize specific deoxynucleotide sequences and cleave the phosphodiester bond between two deoxyribonucleotides at specific sites in each chain. BsaI derived from Bacillus Thermophilus, is a commonly used restriction endonuclease. This product is expressed from E. coli using gene recombination technology, our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

BenzoNuclease® is a recombinant endonuclease derived from Serratia marcescensa. The protein is expressed in Escherichia coli (E. coli). BenzoNuclease® has been shown to exert a broad spectrum of substrate specificity to degrade both DNA and RNA—whether single-stranded, double-stranded, linear, circular or supercoiled. No base preference is observed. As with all endonucleases, BenzoNuclease® hydrolyzes internal phosphodiester bonds present between the nucleotides. Upon complete digestion, all free nucleic acids present in solution are reduced to 5’-monophosphate-terminated oligonucleotides, which are three to five bases in length. In scientific research, BenzoNuclease® can be used as the preferred enzyme preparation for supernatant of cultured cells and viscosity removal of cell lysate, which can remove nucleic acid interference and improve the subsequent protein purification or functional research. At the same time in the vaccine industry, protein and polysaccharide pharmaceutical industry, used to remove the host residual nucleic acid, greatly reduce the vaccine and protein products of nucleic acid pollution, reduce the processing steps, improve the yield of products. This product is expressed by large-scale fermentation of E. coli, and is produced with raw materials of medicinal specifications. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials. FDA DMF {{GMP-1707_application_3.png}} [[GMP-1707_application_3.png]]

Different grades and packaging are available.

BenzoNuclease® is a recombinant endonuclease derived from Serratia marcescensa. The protein is expressed in Escherichia coli (E. coli). BenzoNuclease® has been shown to exert a broad spectrum of substrate specificity to degrade both DNA and RNA—whether single-stranded, double-stranded, linear, circular or supercoiled. No base preference is observed. As with all endonucleases, BenzoNuclease® hydrolyzes internal phosphodiester bonds present between the nucleotides. Upon complete digestion, all free nucleic acids present in solution are reduced to 5’-monophosphate-terminated oligonucleotides, which are three to five bases in length. In scientific research, BenzoNuclease® can be used as the preferred enzyme preparation for supernatant of cultured cells and viscosity removal of cell lysate, which can remove nucleic acid interference and improve the subsequent protein purification or functional research. At the same time in the vaccine industry, protein and polysaccharide pharmaceutical industry, used to remove the host residual nucleic acid, greatly reduce the vaccine and protein products of nucleic acid pollution, reduce the processing steps, improve the yield of products. This product is expressed by large-scale fermentation of E. coli, and is produced with raw materials of medicinal specifications. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.