Pharmazeutische Inhaltsstoffe

Enterokinase (light chain), highly specifically recognizes the Asp-Asp-Asp-Asp-Lys sequence and hydrolyzes the polypeptides at the C-terminus of Lys. It can convert trypsinogen to trypsin in vivo, and fusion proteins with this recognition sequence can also be cleaved. This product, enterokinase, produced by recombinant yeast secretion and expression system, is of a high purity, high bio-activity, and an excellent stability, which allows a wide range of working condition (4-45℃, pH 4.5-9.5) even keeps a part of bio-activity in the presence of various detergent and denatured agents. This product is from a large scale GMP leveled recombinant enterokinase production via Pichia pastoris expression. Applying pharmaceutical leveled adjuvant and material for production, strictly controlling host protein residues, nucleic acid residues and other impurities, we guarantee manufacture and quality control practice complying to GMP regulation, as well as all the materials traceable.

E. coli Poly (A) Polymerase does not depend on the presence of template, and can catalyze the sequential incorporation of ATP in the form of AMP into the 3´ terminal of RNA, i.e., the addition of polyA tail to the 3´ terminal of RNA. Poly (A) polymerase has A high tail adding efficiency and can add 20 to 200 A bases to the 3´ terminal of RNA. Polyadenylation improves the stability of RNA in cells and enhances the expression efficiency of RNA after transfection or microinjection. Poly(A) tails can be used as primer binding sites for first-strand cDNA synthesis in some applications and can be used for end-labeling or quantification of miRNA. The original enzymes of T7 RNA Transcription Enzyme Mix produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

This product is used for pre-treatment of mRNA capping rate detection samples. Combined with special cleavage probe, 15-30nt samples with 5 'end cap structure can be obtained. This kit uses RNase H and cleavage probe to specifically cut the sample, complete the probe binding and enzyme digestion in one step, and effectively recover the 5 'end enzyme digestion fragment by using the specially optimized precipitation solution. The process is simple and rapid, and the treated sample can be directly used for capping rate detection with LC-MS system. The specially designed cleavage probe can effectively reduce the formation of secondary cleavage products and contribute to the analysis of subsequent capping rate detection results.