mRNA Raw Materials & Reagents

By using T7 RNA polymerase for In Vitro Transcription (IVT), a large amount of messenger RNA (mRNA) can be prepared for mRNA vaccine drug development and other related studies. During this reaction, double-stranded RNA (dsRNA) were generated due to different reasons such as sequence structure, T7 RNA polymerase and preparation of reaction system. dsRNA is a pathogen-associated molecular pattern (pathogen-associated Molecular pattern) that can be recognized by multiple pattern receptors of cytoplasmic species, triggering activation of immune pathways, leading to translational inhibition and degradation of mRNA vaccines which affects the ultimate safety and effectiveness of the vaccine. Therefore, it is important to identify, quantify and control dsRNA in the process of mRNA vaccine development. This kit uses double antibody sandwich enzyme-linked immunoassay (ELISA) to detect dsRNA, which can detect and quantify dsRNA residue in mRNA with high sensitivity and specificity.

DNase I (Deoxyribonuclease I) was initially isolated from bovine pancreas. It can randomly degrade single stranded or double stranded DNA with equal efficiency, generating oligonucleotides with 5’-P. The bioactivity of DNase I is highly dependent on Ca²⁺, Mg²⁺ or Mn²⁺. In the presence of Mg²⁺, DNase randomly cuts dsDNA; in the presence of Mg²⁺, DNase cleaves both strands of dsDNA at approximately the same site, resulting in a blunt end or a sticky end with 1 or 2 protruding nucleotides.  DNase I is GMP Grade produced in Pichia pastoris. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has completed the DMF record of FDA and passed the HALAL certification.

Different grades and packaging are available.

Different grades and packaging are available.

Different grades and packaging are available.

With S-adenosylmethionine (SAM) as the methyl group base donor, mRNA Cap 2´-O-Methyltransferase can add a methyl group to the 2´-O of the first nucleotide of the Cap0 structure, [m7GpppN1(pN)x-OH(3')], next to the RNA 5´ end. Methylation of capped mRNA from Cap 0 to Cap1 naturally exists in eukaryocyte, where the Cap1 structure shows an important effect for translation, thus for expression as well. This product doesn’t show a reactivity to RNA substrate other than RNA with Cap0 structure. This product is from a large scale GMP leveled recombinant Cap 2’-O-Methyltransferase production via E. coli expression. Applying pharmaceutical leveled adjuvant and material for production, strictly controlling host protein residues, nucleic acid residues and other impurities, we guarantee manufacture and quality control practice complying to GMP regulation, as well as all the materials traceable. This product has completed the DMF record of FDA and passed the HALAL certification.

This product is used for pre-treatment of mRNA capping rate detection samples. Combined with special cleavage probe, 15-30nt samples with 5 'end cap structure can be obtained. This kit uses RNase H and cleavage probe to specifically cut the sample, complete the probe binding and enzyme digestion in one step, and effectively recover the 5 'end enzyme digestion fragment by using the specially optimized precipitation solution. The process is simple and rapid, and the treated sample can be directly used for capping rate detection with LC-MS system. The specially designed cleavage probe can effectively reduce the formation of secondary cleavage products and contribute to the analysis of subsequent capping rate detection results.

This product can be used for pretreatment of mRNA capping rate detection samples. Combined with special cleavage probe, 15-30nt sample restriction fragment with cap structure at 5 'end can be obtained. This kit uses RNase H and cleavage probe to specifically cleave samples, and the probe binding and digestion are completed in one step which is simple and rapid. The biotin-labeled cleavage probe can be adsorbed by streptavidin magnetic beads, and a single enzyme digestion product can be separated after purification. The specially designed cleavage probe can effectively reduce the formation of secondary cleavage products which is convenient for the subsequent analysis of the capping rate detection.

As the effective component in vaccines or drugs for injection into human, application of the raw materials for producing mRNA should comply with relevant terms of the current edition of " the Pharmacopoeia of the People’s Republic of China " and/or conform to general international common criteria. The identification test of the raw material is the confirmation test of the known raw material to confirm whether the raw material stored in the labelled container is the raw material as indicated. Various enzymes are used in the process of in vitro transcription and modification of mRNA. As a protein substance, enzymes can be identified by Dot Immunobinding Assay (DIBA, 2020 edition, Part IV, General Principles 3402). This kit uses polyvinylidene fluoride (PVDF) as the solid phase, carries out antigen-antibody reaction by DIBA, and carries out the identification test of various raw materials. The kit contains six antibodies, anti-T7 RNA Polymerase antibody, anti-Pyrophosphatase Inorganic (Yeast) antibody, anti-RNase Inhibitor antibody, anti-DNase I antibody, anti-Vaccinia Capping Enzyme antibody and anti-mRNA Cap 2´ -O-Methyltransferase antibody. Enzymes for in vitro transcription and modification of mRNA can be identified by specific qualitative analysis. The kit is easy to operate, clear in results, and has the characteristics of high specificity and high durability.

Inorganic Pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates: Applied in nucleic acid amplification experiments, PPase can hydrolyze the inorganic pyrophosphate generated by the polymerization to avoid its inhibition effect to synthesis of RNA strand. Pyrophosphatase, Inorganic (yeast) is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has completed the DMF record of FDA and passed the HALAL certification.

Inorganic Pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates: Applied in nucleic acid amplification experiments, PPase can hydrolyze the inorganic pyrophosphate generated by the polymerization to avoid its inhibition effect to synthesis of RNA strand. In the production process of mRNA vaccine drugs, PPase can increase the production amount of mRNA. According to regulations, various residues should be detected for mRNA vaccine solution, among which, PPase, as a protein product, belongs to the category of protein residue detection item, so quantitative detection of its residues should be carried out. This kit can detect and quantitatively analyze the PPase residues in Mrna solution with high sensitivity and specificity.

10×BsaI Reaction Buffer, GMP Grade is the companion reaction buffer for the restriction endonuclease BsaI.

10×BspQI Reaction Buffer, GMP Grade is the companion reaction buffer for the restriction endonuclease BspQI.

10×Capping Reaction Buffer, GMP Grade is the companion reaction buffer for the Vaccinia Capping Enzyme, GMP Grade and mRNA Cap 2´-O-Methyltransferase, GMP Grade

10×Poly(A) Polymerase Buffer, GMP Grade is the companion reaction buffer for the E. coli Poly(A) Polymerase, GMP Grade and ATP, GMP Grade

10×RNase R Buffer, GMP Grade is a matching reaction buffer for RNase R This product manufacturing processes are following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

10×Transcription Buffer, GMP Grade is the reaction buffer for T7 RNA Polymerase 2.0. This buffer has been optimized to produce 150-250μg of RNA in one reaction. It is also suitable for co-transcription systems, and the co-transcription capping effciency is greater than 95%. The synthesized RNA can be used for downstream applications such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation. 10×Transcription Buffer processes are strictly controlled to ensure the end products free from impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

10×XbaI Reaction Buffer, GMP Grade is a reaction buffer for restriction endonuclease XbaI This product is produced with pharmaceutical specifications of raw materials, and strictly control all kinds of pollution in the production process. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

ATP, short for Adenosine-5'-triphosphate, is an essential component of energy conversion in biological systems and can be used in a variety of related applications in molecular biology, such as kinase reactions, in vitro transcription, linking and phosphorylation. This product is an ATP sodium saline solution with purity ≥96% (HPLC) with a concentration of 10mM±2mM and is free from endonuclease, exonuclease and ribonuclease contamination. ATP processes are strictly controlled to ensure the end products free from impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has passed the HALAL certification.